Lefkippos Attica Technology Park NCSR "Demokritos" 1 article published in JoVE Biology Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease Anna Hadjihambi*1,2, Rallia-Iliana Velliou*3, Panagiotis Tsialios4, Aigli-Ioanna Legaki3, Antonios Chatzigeorgiou*3, Maritina G. Rouchota*4 1The Roger Williams Institute of Hepatology London, Foundation for Liver Research, 2 Using a diet-induced non-alcoholic fatty liver disease (NAFLD) mouse model, we describe the use of novel in vivo micro-computed tomography imaging techniques as a non-invasive method to assess the progression stages of NAFLD, focusing predominantly on the hepatic vascular network due to its significant involvement in NAFLD-related hepatic dysregulation.